The DMEM/F-12 medium is a commonly used basal medium that provides support for the growth of numerous types of cells. The rich blend of this formulation is a result of its composition, which includes DMEM and Ham’s F-12.
Products
DMEM/Ham’s F-12, with L-Glutamine, with 15 mM HEPES
No.
LTLM-B35
Volume:
500 mlAdd to order
Product Specifications
Appearance
Clear red orange solution
CO2 concentration, optimum
5.5%
Storage and shelf life
Store at +2°C to +8°C protected from light.
Once opened, store at 4° C and use within 6-8 weeks
Shipping conditions
Ambient
Formulation
| Components | Concentration mg/L |
| Amino Acids: | |
| Glycine | 18.75 |
| L-Alanine | 4.45 |
| L-Arginine HCl | 147.50 |
| L-Asparagine H2O | 7.50 |
| L-Aspartic Acid | 6.65 |
| L-Cysteine HCl H2O | 17.56 |
| L-Cystine 2 HCl | 31.29 |
| L-Glutamic Acid | 7.35 |
| L-Glutamine | 365.00 |
| L-Histidine HCl H2O | 31.48 |
| L-Isoleucine | 54.47 |
| L-Leucine | 59.05 |
| L-Lysine HCl | 91.25 |
| L-Methionine | 17.24 |
| L-Phenylalanine | 35.48 |
| L-Proline | 17.25 |
| L-Serine | 26.25 |
| L-Threonine | 53.45 |
| L-Tryptophan | 9.02 |
| L-Tyrosine Disodium Salt | 48.10 |
| L-Valine | 52.85 |
| Vitamins: | |
| D-Biotin | 0.0035 |
| Choline Chloride | 8.98 |
| D-Calcium Pantothenate | 2.24 |
| Folic Acid | 2.66 |
| myo-Inositol | 12.60 |
| Nicotinamide | 2.02 |
| Pyridoxine HCl | 0.031 |
| Pyridoxal HCL | 2.00 |
| Riboflavin | 0.219 |
| Thiamine HCl | 2.17 |
| Vitamin B12 | 0.68 |
| Inorganic Salts: | |
| CaCl2 2 H2O | 154.50 |
| CuSO4 5 H2O | 0.0013 |
| Fe(NO3)3 9 H2O | 0.05 |
| FeSO4 7 H2O | 0.417 |
| KCl | 311.80 |
| MgCl2 6 H2O | 61.20 |
| MgSO4 | 48.84 |
| NaCl | 6996.00 |
| NaHCO3 | 1200.00 |
| Na2HPO4 | 71.02 |
| NaH2PO4 | 54.30 |
| ZnSO4 7 H2O | 0.432 |
| Other Components: | |
| D-Glucose | 3151.00 |
| Hypoxanthine | 2.40 |
| HEPES | 3574.50 |
| Linoleic Acid | 0.042 |
| Lipoic Acid | 0.105 |
| Phenol Red Sodium Salt | 8.63 |
| Putrescine 2 HCl | 0.081 |
| Sodium Pyruvate | 55.00 |
| Thymidine | 0.365 |
Precautions and Disclaimer
This product is for research use only.Download:
General information in pdf
The DMEM/F-12 medium is a commonly used basal medium that provides support for the growth of numerous types of cells. The rich blend of this formulation is a result of its composition, which includes DMEM and Ham’s F-12.
