Ham’s F-12 Medium Nutrient Mix was specifically designed for the purpose of growing Chinese Hamster Ovary (CHO) cells and is widely used as a preferred medium for cell growth in the absence of serum.
Products
Ham’s F-12, with L-Glutamine
No.
LTLM-B13
Volume:
500 mlAdd to order
General Information
Appearance
Clear red orange solution
CO2 concentration, optimum
2.5%
Storage and shelf life
Store at +2°C – +8°C protected from light.
Once opened, store at 4°C and use within 6-8 weeks.
Shipping conditions
Ambient
Formulation
| Components | Concentration mg/l |
| Amino Acids | |
| L-Alanine | 9.00 |
| L-Arginine HCl | 211.00 |
| L-Asparagine H2O | 15.01 |
| L-Aspartic Acid | 13.30 |
| L-CysteineHClH2O | 35.00 |
| L-Glutamine | 146.00 |
| Glycine | 7.51 |
| L-Glutamic Acid | 14.70 |
| L-HistidineHClH2O | 20.96 |
| L-Isoleucine | 3.94 |
| L-Leucine | 13.10 |
| L-Lysine HCl | 36.50 |
| L-Methionine | 4.48 |
| L-Phenylalanine | 4.96 |
| L-Proline | 34.50 |
| L-Serine | 10.50 |
| L-Threonine | 11.90 |
| L-Tryptophan | 2.04 |
| L-Tyrosine2Na2H2O | 7.78 |
| L-Valine | 11.70 |
| D-Glucose | 1802.00 |
| Hypoxanthine | 4.08 |
| Linoleic Acid | 0.084 |
| LipoicAcid | 0.21 |
| Phenol Red Sodium Salt | 1.3 |
| Putrescine 2 HCl | 0.151 |
| Sodium Pyruvate | 110.00 |
| Thymidine | 0.70 |
| Vitamins | |
| D-Biotin | 0.0073 |
| Choline chloride | 13.96 |
| D-Calcium Pantothenate | 0.48 |
| Folic Acid | 1.32 |
| myo-Inositol | 18.00 |
| Nicotinamide | 0.037 |
| Pyridoxine HCl | 0.062 |
| Riboflavin | 0.038 |
| Thiamine HCl | 0.34 |
| VitaminB12 | 1.36 |
| Inorganic Salts | |
| Calcium Chloride 2 H2O | 44.10 |
| CupricSulfate5H2O | 0.0025 |
| Iron(II)Sulfate7H2O | 0.834 |
| Magnesium Chloride 6 H2O | 123.00 |
| Potassium Chloride | 224.00 |
| Sodium Bicarbonate | 1176.00 |
| Sodium Chloride | 7599.00 |
| Sodium Phosphate Dibasic Anhydrous | 142.04 |
| ZincSulfate7H2O | 0.863 |
Precautions and Disclaimer
This product is for research use only.Download:
General information in pdf
Ham’s F-12 Medium Nutrient Mix was specifically designed for the purpose of growing Chinese Hamster Ovary (CHO) cells and is widely used as a preferred medium for cell growth in the absence of serum.
