Iscove’s Modified Dulbecco’s Medium (IMDM) is a modified version of DMEM that is particularly well-suited for cultivating cells at high densities. This formulation has been optimized to support the growth of a variety of cell types, including Jurkat, COS-7 and macrophages.
Products
IMDM, with L-Glutamine, without Supplements
No.
LTLM-B14
Volume:
500 mlAdd to order
Product Specifications
CO2 concentration, optimum
7.0%
pH
7.0 – 7.5
Osmolality
260 – 340 mOsm/kg
Colour and Appearance
Clear red orange solution
Endotoxin
< 1 EU/ml (< 0.1 ng/ml)
Cell Culture
Tested
Sterility
Tested
Storage
+2°C°to +8°C protected from light
Formulation
| Components | Concentration mg/l | Molecular Weight g/mol | Concentration mM |
| Amino Acids | |||
| L-Alanine | 25.00 | 89.09 | 0.28 |
| L-Arginine HCl | 84.00 | 210.66 | 0.40 |
| L-Asparagine H2O | 28.40 | 150.13 | 0.19 |
| L-Aspartic Acid | 30.00 | 133.10 | 0.23 |
| L-Cystine 2 HCl | 91.24 | 240.30 | 0.38 |
| L-Glutamic Acid | 75.00 | 147.13 | 0.51 |
| L-Glutamine | 584.00 | 146.15 | 4.00 |
| Glycine | 30.00 | 75.07 | 0.40 |
| L-HistidineHClH2O | 42.00 | 209.63 | 0.20 |
| L-Isoleucine | 105.00 | 131.17 | 0.80 |
| L-Leucine | 105.00 | 131.17 | 0.80 |
| L-Lysine HCl | 146.00 | 182.65 | 0.80 |
| L-Methionine | 30.00 | 149.21 | 0.20 |
| L-Phenylalanine | 66.00 | 165.19 | 0.40 |
| L-Proline | 40.00 | 115.13 | 0.35 |
| L-Serine | 42.00 | 105.09 | 0.40 |
| L-Threonine | 95.00 | 119.12 | 0.80 |
| L-Tryptophan | 16.00 | 204.23 | 0.0783 |
| L-Tyrosine 2 Na | 89.47 | 225.15 | 0.40 |
| L-Valine | 94.00 | 117.15 | 0.80 |
| Vitamins | |||
| D-Biotin | 0.013 | 244.31 | 0.000053 |
| Choline chloride | 4.00 | 139.62 | 0.0286 |
| D-Calcium Pantothenate | 4.00 | 476.53 | 0.0084 |
| Folic Acid | 4.00 | 441.40 | 0.0091 |
| myo-Inositol | 7.20 | 180.16 | 0.0400 |
| Nicotinamide | 4.00 | 122.12 | 0.0328 |
| Pyridoxal HCl | 4.00 | 203.62 | 0.0196 |
| Riboflavin | 0.40 | 376.36 | 0.0011 |
| Thiamine HCl | 4.00 | 337.27 | 0.0119 |
| Vitamin B12 | 0.013 | 1355.37 | 0.0000096 |
| Inorganic Salts | |||
| Calcium Chloride 2 H2O | 219.00 | 147.01 | 1.49 |
| Magnesium Sulfate | 97.67 | 120.37 | 0.81 |
| Potassium Chloride | 330.00 | 74.55 | 4.43 |
| Potassium Nitrate | 0.076 | 101.10 | 0.000752 |
| Sodium Bicarbonate | 3024.00 | 84.01 | 36.00 |
| Sodium Chloride | 4505.00 | 58.44 | 77.09 |
| Sodium Phosphate Monobasic Anhydrous | 109.00 | 119.98 | 0.91 |
| Sodium Selenite | 0.017 | 263.01 | 0.000065 |
| Other Components | |||
| D-Glucose | 4500.00 | 180.16 | 24.98 |
| HEPES | 5958.00 | 238.31 | 25.00 |
| Phenol Red Sodium Salt | 16.0 | 376.36 | 0.04 |
| Sodium Pyruvate | 110.00 | 110.04 | 1.00 |
Precautions and Disclaimer
This product is for research use only. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.Download:
General information in pdf
Iscove’s Modified Dulbecco’s Medium (IMDM) is a modified version of DMEM that is particularly well-suited for cultivating cells at high densities. This formulation has been optimized to support the growth of a variety of cell types, including Jurkat, COS-7 and macrophages.
