McCoy’s 5A Medium is suitable for promoting the growth of various cell types, including primary cells, established cell lines, and explants. Additionally, it is utilized in the generation of viruses in primary cell cultures.
Products
McCoy’s 5A Medium, with L-Glutamine
No.
LTLM-B17
Volume:
500 mlAdd to order
Product Specifications
CO2 concentration, optimum
5.0%
pH
7.0 – 7.5
Osmolality
280 – 350 mOsm/kg
Colour and Appearance
Clear red orange solution
Endotoxin
<1 EU/ml (<0.1 ng/ml)
Cell Culture
Tested
Sterility
Tested
Storage
+2°C to +8°C protected from light
Formulation
| Components | Concentration mg/l | Molecular Weight g/mol | Concentration mM |
| Amino Acids | |||
| L-Alanine | 13.36 | 89.09 | 0.15 |
| L-Arginine HCl | 42.14 | 210.66 | 0.20 |
| L-Asparagine H2O | 45.03 | 150.13 | 0.30 |
| L-Aspartic Acid | 19.97 | 133.10 | 0.15 |
| L-Cystine 2 HCl | 31.75 | 175.63 | 0.18 |
| L-Glutamic Acid | 22.07 | 147.13 | 0.15 |
| L-Glutamine | 219.15 | 146.15 | 1.50 |
| Glycine | 7.51 | 75.07 | 0.10 |
| L-HistidineHClH2O | 20.96 | 209.63 | 0.10 |
| L-Hydroxyproline | 19.67 | 131.13 | 0.15 |
| L-Isoleucine | 39.36 | 131.17 | 0.30 |
| L-Leucine | 39.36 | 131.17 | 0.30 |
| L-Lysine HCl | 36.54 | 182.65 | 0.20 |
| L-Methionine | 14.92 | 149.21 | 0.10 |
| L-Phenylalanine | 16.52 | 165.19 | 0.10 |
| L-Proline | 17.27 | 115.13 | 0.15 |
| L-Serine | 26.28 | 105.09 | 0.25 |
| L-Threonine | 17.87 | 119.12 | 0.15 |
| L-Tryptophan | 3.06 | 204.23 | 0.015 |
| L-Tyrosine 2 Na | 26.10 | 225.15 | 0.12 |
| L-Valine | 17.57 | 117.15 | 0.15 |
| Vitamins | |||
| 4-Aminobenzoic Acid | 1.00 | 137.14 | 0.0073 |
| Ascorbic Acid | 0.56 | 176.12 | 0.0032 |
| D-Biotin | 0.20 | 244.31 | 0.0008 |
| Choline chloride | 5.00 | 139.62 | 0.0358 |
| D-Calcium Pantothenate | 0.20 | 476.53 | 0.0004 |
| Folic Acid | 10.00 | 441.40 | 0.0227 |
| myo-Inositol | 36.00 | 180.16 | 0.20 |
| Nicotinamide | 0.50 | 122.12 | 0.0041 |
| Nicotinic Acid | 0.50 | 123.11 | 0.0041 |
| Pyridoxal HCl | 0.50 | 203.62 | 0.0025 |
| Pyridoxine HCl | 0.50 | 203.62 | 0.0025 |
| Riboflavin | 0.20 | 376.36 | 0.0005 |
| Thiamine HCl | 0.20 | 337.27 | 0.0006 |
| Vitamin B12 | 2.00 | 1355.37 | 0.0015 |
| Inorganic Salts | |||
| Calcium Chloride 2 H2O | 132.00 | 147.01 | 0.90 |
| Magnesium Sulfate | 97.67 | 120.37 | 0.81 |
| Potassium Chloride | 400.00 | 74.55 | 5.37 |
| Sodium Bicarbonate | 2200.00 | 84.01 | 26.19 |
| Sodium Chloride | 6460.00 | 58.44 | 110.54 |
| Sodium Phosphate Monobasic Anhydrous | 504.00 | 119.98 | 4.20 |
| Other Components | |||
| D-Glucose | 3000.00 | 180.16 | 16.65 |
| L-Glutathione Reduced | 0.50 | 307.32 | 0.0016 |
| Meat Peptone | 600.00 | - | - |
| Phenol Red Sodium Salt | 11.0 | 376.36 | 0.03 |
Precautions and Disclaimer
This product is for research use only. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.Download:
General information in pdf
McCoy’s 5A Medium is suitable for promoting the growth of various cell types, including primary cells, established cell lines, and explants. Additionally, it is utilized in the generation of viruses in primary cell cultures.
