Medium 199 (M199) was specifically created to support the growth of primary explants and is ideal for various applications, including vaccine production, virology and other in vitro cell culture experiments.
Products
Medium 199 with Earle’s Salts, with L-Glutamine
No.
LTLM-B16
Volume:
500 mlAdd to order
Product Specifications
CO2 concentration, optimum
5.0%
pH
7.0 – 7.5
Osmolality
280 – 340 mOsm/kg
Colour and Appearance
Clear red orange solution
Endotoxin
< 1 EU/ml (< 0.1 ng/ml)
Cell Culture
Tested
Sterility
Tested
Storage
+2°C° to +8°C protected from light
Formulation
| Components | Concentration mg/l | Molecular Weight g/mol | Concentration mM |
| Amino Acids | |||
| L-Alanine | 50.00 | 89.09 | 0.56 |
| L-Arginine HCl | 70.00 | 210.66 | 0.33 |
| L-Aspartic Acid | 60.00 | 133.10 | 0.45 |
| L-Cysteine HCl H2O | 0.11 | 176.00 | 0.0006 |
| L-Cystine 2 HCl | 26.00 | 240.30 | 0.11 |
| L-Glutamic Acid | 133.60 | 147.13 | 0.91 |
| L-Glutamine | 100.00 | 146.15 | 0.68 |
| Glycine | 50.00 | 75.07 | 0.67 |
| L-Histidine HCl H2O | 21.88 | 209.63 | 0.10 |
| L-Hydroxy-L-Proline | 10.00 | 131.00 | 0.08 |
| L-Isoleucine | 40.00 | 131.17 | 0.30 |
| L-Leucine | 120.00 | 131.17 | 0.91 |
| L-Lysine HCl | 70.00 | 182.65 | 0.38 |
| L-Methionine | 30.00 | 149.21 | 0.20 |
| L-Phenylalanine | 50.00 | 165.19 | 0.30 |
| L-Proline | 40.00 | 115.13 | 0.35 |
| L-Serine | 50.00 | 105.09 | 0.48 |
| L-Threonine | 60.00 | 119.12 | 0.50 |
| L-Tryptophan | 20.00 | 204.23 | 0.10 |
| L-Tyrosine 2 Na | 57.66 | 225.15 | 0.26 |
| L-Valine | 50.00 | 117.15 | 0.43 |
| Vitamins | |||
| Ascorbic Acid | 0.056 | 176.12 | 3.18E-4 |
| D-Biotin | 0.01 | 244.31 | 4.09E-5 |
| Choline chloride | 0.50 | 139.62 | 0.0036 |
| D-Calcium Pantothenate | 0.01 | 476.53 | 2.10E-5 |
| Folic Acid | 0.01 | 441.40 | 2.27E-5 |
| Menadione Sodium Bisulfite | 0.016 | 276.24 | 5.79E-5 |
| myo-Inositol | 0.05 | 180.16 | 2.78E-4 |
| Nicotinamide | 0.025 | 122.12 | 2.05E-4 |
| Nicotinic Acid | 0.025 | 123.11 | 2.03E-4 |
| Paraaminobenzoic Acid | 0.05 | 137.14 | 3.65E-4 |
| Pyridoxal HCl | 0.025 | 203.62 | 1.23E-4 |
| Pyridoxine HCl | 0.025 | 205.64 | 1.22E-4 |
| Riboflavin | 0.01 | 376.36 | 2.66E-5 |
| Thiamine HCl | 0.01 | 337.27 | 2.96E-5 |
| Vitamin A (Acetate) | 0.14 | 328.49 | 4.26E-4 |
| Vitamin D2 (Ergocalciferol) | 0.10 | 396.65 | 2.52E-4 |
| (±)-α-Tocopherol Phosphate 2Na | 0.01 | 554.65 | 1.80E-5 |
| Inorganic Salts | |||
| Calcium Chloride 2 H2O | 265.00 | 147.01 | 1.80 |
| Ferric Nitrate 9 H2O | 0.72 | 404.00 | 0.0018 |
| Magnesium Sulfate | 97.67 | 120.37 | 0.81 |
| Potassium Chloride | 400.00 | 74.55 | 5.37 |
| Sodium Bicarbonate | 1250.00 | 84.01 | 17.88 |
| Sodium Chloride | 6800.00 | 58.44 | 116.36 |
| Sodium Phosphate Monobasic Anhydrous | 122.00 | 119.98 | 1.02 |
| Other Components | |||
| 2-Deoxy-D-Ribose | 0.50 | 134.13 | 0.004 |
| Adenine Sulfate | 10.00 | 184.17 | 0.05 |
| Adenosine 5‘-Phosphate | 0.24 | 365.24 | 6.57E-4 |
| Adenosine 5‘-Triphosphate 2Na | 1.00 | 551.14 | 0.002 |
| Cholesterol | 0.20 | 386.65 | 5.17E-4 |
| D-Glucose | 1000.00 | 180.16 | 5.55 |
| L-Glutathione (reduced) | 0.05 | 307.32 | 1.63E-4 |
| Guanine | 0.30 | 151.13 | 0.002 |
| Hypoxanthine | 0.30 | 136.11 | 0.002 |
| Phenol Red Sodium Salt | 21.3 | 376.36 | 0.057 |
| D-Ribose | 0.50 | 150.13 | 0.003 |
| Sodium Acetate | 50.00 | 82.00 | 0.61 |
| Thymine | 0.30 | 126.11 | 0.002 |
| Tween® 80 | 20.00 | - | Infinity |
| Uracil | 0.30 112.09 0.003 | 112.09 | 0.003 |
| Xanthine Na | 0.34 | 174.09 | 0.002 |
Precautions and Disclaimer
This product is for research use only. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.Download:
General information in pdf
Medium 199 (M199) was specifically created to support the growth of primary explants and is ideal for various applications, including vaccine production, virology and other in vitro cell culture experiments.
