MEM is a commonly utilized cell culture medium that can support the growth of various cell types, whether they are adherent or suspension.
Products
MEM Alpha Modification, w/o L-Glutamine, with Nucleosides
No.
LTLM-B19
Volume:
500 mlAdd to order
Product Specifications
CO2 concentration, optimum
5.0 %
pH
7.0 – 7.5
Osmolality
260 – 340 mOsm/kg
Colour and Appearance
Clear red orange solution
Endotoxin
<1 EU/ml (<0.1 ng/ml)
Cell Culture
Tested
Sterility
Tested
Storage
+2°C to +8°C protected from light, do not freeze!
Formulation
| Components | Concentration mg/l | Molecular Weight g/mol | Concentration mM |
| Amino Acids | |||
| L-Alanine | 25.00 | 89.09 | 0.28 |
| L-Arginine · HCl | 126.00 | 210.66 | 0.60 |
| L-Asparagine · H2O | 50.00 | 150.13 | 0.33 |
| L-Aspartic Acid | 30.00 | 133.10 | 0.23 |
| L-Cysteine · HCl · H2O | 100.00 | 175.63 | 0.57 |
| L-Cystine 2 HCl | 31.30 | 313.22 | 0.10 |
| L-Glutamic Acid | 75.00 | 147.13 | 0.51 |
| Glycine | 50.00 | 75.07 | 0.67 |
| L-Histidine · HCl · H2O | 42.00 | 209.63 | 0.20 |
| L-Isoleucine | 52.00 | 131.17 | 0.40 |
| L-Leucine | 52.00 | 131.17 | 0.40 |
| L-Lysine HCl | 72.50 | 182.65 | 0.40 |
| L-Methionine | 15.00 | 149.21 | 0.10 |
| L-Phenylalanine | 32.00 | 165.19 | 0.19 |
| L-Proline | 40.00 | 115.13 | 0.35 |
| L-Serine | 25.00 | 105.09 | 0.24 |
| L-Threonine | 48.00 | 119.12 | 0.40 |
| L-Tryptophan | 10.00 | 204.23 | 0.05 |
| L-Tyrosine · 2 Na · H2O | 26.10 | 225.15 | 0.12 |
| L-Valine | 46.00 | 117.15 | 0.39 |
| Vitamins | |||
| Ascorbic Acid | 50.00 | 176.12 | 0.28 |
| D-Biotin | 0.10 | 244.31 | 0.0004 |
| Choline chloride | 1.00 | 139.62 | 0.0072 |
| D-Calcium Pantothenate | 1.00 | 476.53 | 0.0021 |
| Folic Acid | 1.00 | 441.40 | 0.0023 |
| myo-Inositol | 2.00 | 180.16 | 0.01 |
| Nicotinamide | 1.00 | 122.12 | 0.0082 |
| Pyridoxal HCl | 1.00 | 203.62 | 0.0049 |
| Riboflavin | 0.10 | 376.36 | 0.0003 |
| Thiamine HCl | 1.00 | 337.27 | 0.0030 |
| Vitamin B12 | 1.36 | 1355.37 | 0.0010 |
| Inorganic Salts | |||
| CaCl2 · 2 H2O | 200.00 | 147.01 | 1.80 |
| MgSO4 (anhydrous) | 97.67 | 120.37 | 0.81 |
| KCl | 400.00 | 74.55 | 5.37 |
| NaHCO3 | 2200.00 | 84.01 | 26.19 |
| NaCl | 6800.00 | 58.44 | 116.36 |
| Na2HPO4 (anhydrous) | 122.00 | 119.98 | 1.02 |
| Other Components | |||
| Adenosine | 10.00 | ||
| Cytidine | 10.00 | ||
| Guanosine | 10.00 | ||
| Uridine | 10.00 | ||
| 2’-Deoxyadenosine | 10.00 | ||
| 2’-Deoxycytidine · HCl | 10.00 | ||
| 2’-Deoxyguanosine | 10.00 | ||
| Thymidine | 10.00 | ||
| D-Glucose | 1000.00 | 180.16 | 5.55 |
| Lipoic Acid | 0.20 | 206.33 | 0.001 |
| Phenol Red · Na | 11.0 | 376.36 | 0.03 |
| Sodium Pyruvate | 110.00 | 110.04 | 1.00 |
Precautions and Disclaimer
This product is for research use only. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.Download:
General information in pdf
MEM is a commonly utilized cell culture medium that can support the growth of various cell types, whether they are adherent or suspension.
