PlasmidCap Persefose HP is a thiophilic affinity resin made by fixing sulfur-containing compound 2-mercapyridine on high resolution crosslinked agarose. Its optimized ligand density has appropriate affinity with superhelix DNA, and fine particle microspheres can improve the load of superhelix DNA with larger molecular weight. The principle of thiophilic affinity is to use the interaction between electron donor and electron acceptor to separate and purify biomolecules. This force is strengthened in a high salt environment and weakened in a low salt environment.
Products
PlasmidCap Persefose HP
No.
181-00025 / 25ml
181-00100 / 100ml
181-00500 / 500ml
181-01000 / 1L
181-05000 / 5L
181-10000 / 10L
181-20000 / 20L
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Chromatography resin parameters
Resin type
DNA affinity chromatography
Matrix
High degree cross-linking agarose
Functional group
2-mercaptopyridine
Median particle size
34
Ligand density
~3.5 mg/ml
Dynamic binding capacity
>2.0 mg plasmid/ml
Recommended flow rate
50~120 cm/h
Maximum working pressure
3 bar
Working temperature
15–30°C
Chemical resistance
| pH stability* | 3–11 (working), 2–13 (CIP) |
| Ionic strength | All commonly used aqueous buffers, 1M Acetic acid, 30% isopropanol**, 70% ethanol**, 0.1M NaOH |
** v/v, volume ratio
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PlasmidCap Persefose HP is a thiophilic affinity resin made by fixing sulfur-containing compound 2-mercapyridine on high resolution crosslinked agarose. Its optimized ligand density has appropriate affinity with superhelix DNA, and fine particle microspheres can improve the load of superhelix DNA with larger molecular weight. The principle of thiophilic affinity is to use the interaction between electron donor and electron acceptor to separate and purify biomolecules. This force is strengthened in a high salt environment and weakened in a low salt environment.
